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gal1 promoter  (Addgene inc)


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    Structured Review

    Addgene inc gal1 promoter
    Gal1 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gal1+promoter/pJRL2+GAL1(TATA-A1T6)pr+VYFP+(EB%231573)+(Plasmid+%2314849)/bio_rxiv__2024__12__24__630284-221-6-31
    Average 93 stars, based on 1 article reviews
    gal1 promoter - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Accessible Type 2 diabetes medication through stable expression of Exendin-4 in Saccharomyces cerevisiae
    Article Snippet: .. Briefly, the S. cerevisiae Modular Cloning library (MoClo) was used to include a GAL1 promoter (Addgene#65137) and PGK1 terminator (Addgene#65161) ( ) for both constructs. ..

    Article Title: Accessible Type 2 diabetes medication through stable expression of Exendin-4 in Saccharomyces cerevisiae
    Article Snippet: 2.2 Exendin-4 in Saccharomyces cerevisiae Two non-replicative fragments (pLEU and pTRP) used for chromosomal integration were prepared in E. coli and following the design outlined in Figure 3. .. Briefly, the S. cerevisiae Modular Cloning library (MoClo) was used to include a GAL1 promoter (Addgene#65137) and PGK1 terminator (Addgene#65161) (Lee et al., 2015) for both constructs. .. The codon-optimized Ex-4 sequence (Integrated DNA Technologies, 2023) includes a C-terminal half-life extender comprising a 10X repeat of VPGVG (Jung et al., 2019).

    Construct:

    Article Title: Accessible Type 2 diabetes medication through stable expression of Exendin-4 in Saccharomyces cerevisiae
    Article Snippet: .. Briefly, the S. cerevisiae Modular Cloning library (MoClo) was used to include a GAL1 promoter (Addgene#65137) and PGK1 terminator (Addgene#65161) ( ) for both constructs. ..

    Article Title: Improving Industrially Relevant Phenotypic Traits by Engineering Chromosome Copy Number in Saccharomyces pastorianus
    Article Snippet: .. Primers were ordered at Sigma Aldrich ( ). pART001 was constructed using the NEBuilder ® HiFi DNA Assembly method with the backbone of pUG-amdSYM, amplified using primers 8624 & 8556, and the GAL1 promoter of pAG426GAL (pAG426GAL Addgene plasmid # 14155; http://n2t.net/addgene:14155 ) ( ) that was amplified using primers 8623 & 8436. .. Genomic DNA of CBS 1483 was extracted with the YeaStarTM Genomic DNA kit (Zymo Research, Irvine, CA, United States) according to Protocol I supplied by the manufacturer.

    Article Title: Accessible Type 2 diabetes medication through stable expression of Exendin-4 in Saccharomyces cerevisiae
    Article Snippet: 2.2 Exendin-4 in Saccharomyces cerevisiae Two non-replicative fragments (pLEU and pTRP) used for chromosomal integration were prepared in E. coli and following the design outlined in Figure 3. .. Briefly, the S. cerevisiae Modular Cloning library (MoClo) was used to include a GAL1 promoter (Addgene#65137) and PGK1 terminator (Addgene#65161) (Lee et al., 2015) for both constructs. .. The codon-optimized Ex-4 sequence (Integrated DNA Technologies, 2023) includes a C-terminal half-life extender comprising a 10X repeat of VPGVG (Jung et al., 2019).

    Clone Assay:

    Article Title: Metabolic regulation of misfolded protein import into mitochondria
    Article Snippet: .. FlucSM- HA- GFP11 and FUSP525L- HA- GFP11 under GAL1 promoter were cloned from plasmids from our previous study (Ruan et al., 2017) and plasmid 416Gal- FUS- P525L- YFP (Addgene plasmid #29628). .. FlucWT- HA- GFP11 and FlucDM- HA- GFP11 plasmids were constructed using sitedirected mutagenesis kit (NEB) based on FlucSM- HA- GFP11.

    Article Title: Metabolic regulation of misfolded protein import into mitochondria
    Article Snippet: .. FlucSM-HA-GFP 11 and FUS P525L -HA-GFP 11 under GAL1 promoter were cloned from plasmids from our previous study ( ) and plasmid 416Gal-FUS-P525L-YFP, a gift from Aaron Gitler (Addgene plasmid #29628). .. FlucWT-HA-GFP 11 and FlucDM-HA-GFP 11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP 11 .

    Article Title: Metabolic regulation of misfolded protein import into mitochondria
    Article Snippet: .. FlucSM-HA-GFP 11 and FUS P525L -HA-GFP 11 under GAL1 promoter were cloned from plasmids from our previous study ( ) and plasmid 416Gal-FUS-P525L-YFP (Addgene plasmid #29628). .. FlucWT-HA-GFP 11 and FlucDM-HA-GFP 11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP 11 .

    Plasmid Preparation:

    Article Title: Metabolic regulation of misfolded protein import into mitochondria
    Article Snippet: .. FlucSM- HA- GFP11 and FUSP525L- HA- GFP11 under GAL1 promoter were cloned from plasmids from our previous study (Ruan et al., 2017) and plasmid 416Gal- FUS- P525L- YFP (Addgene plasmid #29628). .. FlucWT- HA- GFP11 and FlucDM- HA- GFP11 plasmids were constructed using sitedirected mutagenesis kit (NEB) based on FlucSM- HA- GFP11.

    Article Title: Improving Industrially Relevant Phenotypic Traits by Engineering Chromosome Copy Number in Saccharomyces pastorianus
    Article Snippet: .. Primers were ordered at Sigma Aldrich ( ). pART001 was constructed using the NEBuilder ® HiFi DNA Assembly method with the backbone of pUG-amdSYM, amplified using primers 8624 & 8556, and the GAL1 promoter of pAG426GAL (pAG426GAL Addgene plasmid # 14155; http://n2t.net/addgene:14155 ) ( ) that was amplified using primers 8623 & 8436. .. Genomic DNA of CBS 1483 was extracted with the YeaStarTM Genomic DNA kit (Zymo Research, Irvine, CA, United States) according to Protocol I supplied by the manufacturer.

    Article Title: Metabolic regulation of misfolded protein import into mitochondria
    Article Snippet: .. FlucSM-HA-GFP 11 and FUS P525L -HA-GFP 11 under GAL1 promoter were cloned from plasmids from our previous study ( ) and plasmid 416Gal-FUS-P525L-YFP, a gift from Aaron Gitler (Addgene plasmid #29628). .. FlucWT-HA-GFP 11 and FlucDM-HA-GFP 11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP 11 .

    Article Title: Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters
    Article Snippet: .. Vectors pYD1 The vector containing a GAL1 promoter and an Aga2 gene, used in the S. cerevisiae a-agglutinin surface display system (RRID: Addgene_73447) [1] pYD1-GDH1 pYD1 derived with an insertion GDH1 gene This study pYD1-CHO1 pYD1 derived with an insertion CHO1 gene This study pYD1-CHO1-PASx1 pYD1-CHO1 derived with a modified linker which added one PAS sequence to the original GS linker This study pYD1-CHO1-PASx2 pYD1-CHO1 derived with a modified linker which added two PAS sequences to the original GS linker This study ..

    Article Title: Metabolic regulation of misfolded protein import into mitochondria
    Article Snippet: .. FlucSM-HA-GFP 11 and FUS P525L -HA-GFP 11 under GAL1 promoter were cloned from plasmids from our previous study ( ) and plasmid 416Gal-FUS-P525L-YFP (Addgene plasmid #29628). .. FlucWT-HA-GFP 11 and FlucDM-HA-GFP 11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP 11 .

    Article Title: Elucidation of gene clusters underlying withanolide biosynthesis in ashwagandha through yeast metabolic engineering
    Article Snippet: Candidate genes were cloned into yeast integration vectors from the EasyClone-MarkerFree Vector set , a gift from Irina Borodina (Addgene kit #1000000098), using NEBuilder HiFi Assembly cloning (NEB). .. Candidate genes were placed under the GAL1 promoter, amplified from pYTK030 from the MoClo-YTK plasmid kit , or under the bidirectional GAL1/10 promoter, amplified from pJK372 (gift from T. J. Kappock; Addgene plasmid # 73597; http://n2t.net/addgene:73597 ; RRID:Addgene_73597). .. The MoClo-YTK plasmid kit was a gift from John Dueber (Addgene kit # 1000000061).

    Amplification:

    Article Title: Improving Industrially Relevant Phenotypic Traits by Engineering Chromosome Copy Number in Saccharomyces pastorianus
    Article Snippet: .. Primers were ordered at Sigma Aldrich ( ). pART001 was constructed using the NEBuilder ® HiFi DNA Assembly method with the backbone of pUG-amdSYM, amplified using primers 8624 & 8556, and the GAL1 promoter of pAG426GAL (pAG426GAL Addgene plasmid # 14155; http://n2t.net/addgene:14155 ) ( ) that was amplified using primers 8623 & 8436. .. Genomic DNA of CBS 1483 was extracted with the YeaStarTM Genomic DNA kit (Zymo Research, Irvine, CA, United States) according to Protocol I supplied by the manufacturer.

    Article Title: Elucidation of gene clusters underlying withanolide biosynthesis in ashwagandha through yeast metabolic engineering
    Article Snippet: Candidate genes were cloned into yeast integration vectors from the EasyClone-MarkerFree Vector set , a gift from Irina Borodina (Addgene kit #1000000098), using NEBuilder HiFi Assembly cloning (NEB). .. Candidate genes were placed under the GAL1 promoter, amplified from pYTK030 from the MoClo-YTK plasmid kit , or under the bidirectional GAL1/10 promoter, amplified from pJK372 (gift from T. J. Kappock; Addgene plasmid # 73597; http://n2t.net/addgene:73597 ; RRID:Addgene_73597). .. The MoClo-YTK plasmid kit was a gift from John Dueber (Addgene kit # 1000000061).

    Derivative Assay:

    Article Title: Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters
    Article Snippet: .. Vectors pYD1 The vector containing a GAL1 promoter and an Aga2 gene, used in the S. cerevisiae a-agglutinin surface display system (RRID: Addgene_73447) [1] pYD1-GDH1 pYD1 derived with an insertion GDH1 gene This study pYD1-CHO1 pYD1 derived with an insertion CHO1 gene This study pYD1-CHO1-PASx1 pYD1-CHO1 derived with a modified linker which added one PAS sequence to the original GS linker This study pYD1-CHO1-PASx2 pYD1-CHO1 derived with a modified linker which added two PAS sequences to the original GS linker This study ..

    Modification:

    Article Title: Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters
    Article Snippet: .. Vectors pYD1 The vector containing a GAL1 promoter and an Aga2 gene, used in the S. cerevisiae a-agglutinin surface display system (RRID: Addgene_73447) [1] pYD1-GDH1 pYD1 derived with an insertion GDH1 gene This study pYD1-CHO1 pYD1 derived with an insertion CHO1 gene This study pYD1-CHO1-PASx1 pYD1-CHO1 derived with a modified linker which added one PAS sequence to the original GS linker This study pYD1-CHO1-PASx2 pYD1-CHO1 derived with a modified linker which added two PAS sequences to the original GS linker This study ..

    Sequencing:

    Article Title: Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters
    Article Snippet: .. Vectors pYD1 The vector containing a GAL1 promoter and an Aga2 gene, used in the S. cerevisiae a-agglutinin surface display system (RRID: Addgene_73447) [1] pYD1-GDH1 pYD1 derived with an insertion GDH1 gene This study pYD1-CHO1 pYD1 derived with an insertion CHO1 gene This study pYD1-CHO1-PASx1 pYD1-CHO1 derived with a modified linker which added one PAS sequence to the original GS linker This study pYD1-CHO1-PASx2 pYD1-CHO1 derived with a modified linker which added two PAS sequences to the original GS linker This study ..



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    Image Search Results


    Cells and plasmids used in this study

    Journal: Journal of Lipid Research

    Article Title: The antidepressant drug sertraline is a novel inhibitor of yeast Pah1 and human lipin 1 phosphatidic acid phosphatases

    doi: 10.1016/j.jlr.2024.100711

    Figure Lengend Snippet: Cells and plasmids used in this study

    Article Snippet: pYES2 , High-copy number E.coli /yeast shuttle vector with URA3 and GAL1 promoter , Thermo Fisher Scientific.

    Techniques: Plasmid Preparation, Expressing, Sequencing